Figure 7.

Bim promoter region is subject to epigenetic changes during βAR signalling. (a) Bisulphite sequencing analysis of RAMOS, LK-2 and LK-2 cells expressing full-length CBP using primers flanking the region between −1513 and −948 (from the transcription initiation site). The numbers shown are the potential CpG methylation sites. (b) ChIP analysis of the Bim promoter in MEFs expressing PKACα using antibodies as indicated. (c) A model for Bim induction by cAMP/PKA pathway. PKA activation of CBP bound to the Bim promoter through its interaction with c-Myc results in histone 4 acetylation, leading to Bim transcriptional induction