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. 2013 Jun 1;126(11):2446–2458. doi: 10.1242/jcs.119214

Fig. 4.

Fig. 4.

MiR146b knockdown reduced the alveolar luminal progenitor cell number when grown in Matrigel®. (A) Quantitative PCR of the PMECs at 24 hours post-transfection (before growth on Matrigel®) and after 7 days on Matrigel. (B) Immunofluorescent images of the solid and hollow acinar structures. The nucleus is counterstained with DAPI (blue). (C) The solid organoid- and hollow acinus-forming efficiencies of PMECs transfected with the miR146b-LNA inhibitor (miR146b KD) compared with non-silencing controls (NS) and non-transfected cells (NT). The cells were cultured on Matrigel for 7 days, either with or without prolactin treatment (n = 3, P<0.05). (D,E) RT-qPCR of the CD-derived alveolar progenitor cells (D), and primary mammary epithelial cells (E) transfected with the miR146b-LNA inhibitor (miR146b KD) compared with non-silencing controls (NS) and non-transfected cells (NT). The cells were cultured on Matrigel for 72 hours, with prolactin treatment. Cells were recovered and analyzed for β-casein expression by qPCR (n = 3, *P<0.05 compared with other cells). Data are means ± s.e.m.