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. Author manuscript; available in PMC: 2013 Jun 12.
Published in final edited form as: Nature. 2011 Oct 2;478(7370):529–533. doi: 10.1038/nature10509

Figure 2. I-BET151 selectively and potently inhibits MLL-fusion leukaemic cell lines in vitro.

Figure 2

(A) Human leukaemia cell lines tested using I-BET151. (B) Clonogenic assays performed in the presence of DMSO or I-BET151. (C) Haematopoietic progenitors were isolated from mouse bone marrow and retrovirally transformed with MLL-ENL or MLL-AF9. These cells were used in both proliferation and clonogenic assays. (D) Apoptosis was assessed by FACS analysis following 72 hours incubation with DMSO or I-BET151. (E) Cell cycle progression was assessed by FACS analysis 24 hours after incubation with DMSO or I-BET151. Bar graphs are represented as the mean and error bars reflect standard deviation of results derived from triplicate experiments.