Figure 1. IFT transports FMG1-B.
(A) (Top) Simultaneous tracking of anti-FMG1-B beads (red) and IFT27-GFP (green). (Bottom) Kymographs show that bead motility colocalizes with IFT trains during processive runs. Between the runs, the bead transiently attaches to and detaches from IFT trains. The white arrows indicate the IFT trains transporting the bead. (B) Two-dimensional Gaussian fitting of the bead and IFT trains show that bead motility correlates strongly (>0.99) with the movement of individual anterograde (green shaded region) and retrograde (blue shaded region) IFT trains. The bead moves at similar speeds to IFT trains in both anterograde and retrograde directions. (C) Comparison of distances from beads to the closest IFT train moving in the same direction (grey bars) to the predicted distribution without correlation (null hypothesis, black line). Kolmogorov-Smirnov statistics indicate that bead and IFT train positions strongly correlate with each other. (D) A model for IFT particles transporting FMG1-B. The bead is attached to FMG1-B in the flagellar membrane through antibody linkages (not to scale).
Figure 1—figure supplement 1. Additional examples of simultaneous tracking of bead motility and IFT.
