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Simultaneous tracking of IFT27-GFP and bead motility. At t = 3 s, the microscope stage was moved to bring the flagellum underneath the trapped bead. An IFT train stalls at the trap position (t = 5–8 s) and stays adhered to the bead until the bead escapes the trap (t = 10 s). PSD signal shows a plateau and then decreases as the bead moves further away from the trap center, as shown in Author response image 1.
