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. 2013 Jan 7;12(6):2449–2457. doi: 10.1021/pr301011r

Figure 1.

Figure 1

IonPac A24 shows high orthogonality, reproducibility and linearity. (A) 2D retention maps of peptides of a 6-protein mix digest show that elution with up to 1 M NaCl in absence of organic solvent leads to high orthogonality for AS24 (ultralow hydrophobicity), but part and full retention of peptides by AS11-HC (medium hydrophobicity) and AS15 (high hydrophobicity), respectively. (B) Addition of 25% acetonitrile (AcN) into the elution buffer enhances elution from AS11-HC and AS15. (C) Three replicate injections on AS24 over the course of 48 h show high reproducibility. (D) Increasing amounts of protein digest loaded on AS24 show good linear behavior without peak broadening.