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. 2013 Jan 7;12(6):2449–2457. doi: 10.1021/pr301011r

Figure 3.

Figure 3

Comparison of protein and peptide identifications from off-line separation by either hSAX/RP or RP/RP and LC–MS experiments. (A) The high orthogonality of the hSAX approach is exemplified by the identification of more than 3000 proteins in 16 out of 34 fractions, outperforming RP/RP approach (6 out of 34 fractions). (B) Moreover, the hSAX was comparable to the RP/RP approach in separating peptides in a single fraction (69% of RP peptides were present in one fraction compared to 55% for the hSAX approach), which is beneficial for quantitative proteomics experiments.