Skip to main content
. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: J Mol Cell Cardiol. 2013 Apr 20;60:121–128. doi: 10.1016/j.yjmcc.2013.04.014

Figure 2. Ca influx and SR Ca load remain unchanged in Pak1−/− VMs.

Figure 2

(A) Current voltage relationship of ICa,L in WT and Pak1−/− VMs obtained in the whole cell patch configuration from a holding potential of −50 mV (−40 to +40 mV), in 10 mV increments. (B) Representative current traces from WT and Pak1−/− VMs during a voltage pulse to 10 mV. (C) ICa,L at −10 mV and (D) the amplitude of the caffeine (10 mmol/L) induced Ca transient were not significantly different in WT and Pak1−/− VMs. (F) The propagation velocity of spontaneous Ca waves and their amplitude (E) was comparable in WT and Pak1−/− VMs. (G) Western blotting analysis did not reveal differences in the protein expression of SERCA2a or Caf1.2 in 4 and 6 month old Pak1−/− VMs. α-actinin is shown as a loading control.