Figure 7. In Vivo Antiviral Macrophage Response Is Impaired in ABX Mice after LCMV or Influenza Virus Infection.

(A and B) CNV or ABX mice were inoculated with LCMV (T1b) or PBS i.v. and (A) splenocytes at 6 hr p.i. or (B) PECs at 12 hr p.i. were immediately fixed to preserve the in vivo STAT1 phosphorylation status of macrophages.

(C) CNV or ABX mice were infected with influenza virus (PR8-GP33). At d3 p.i., alveolar macrophages were sorted from the BAL and in vivo induction of antiviral defense genes was assessed by RT-PCR. Gene expression displayed as fold induction over naive alveolar macrophages from CNV mice. Data representative of two independent experiments with n = 3–5 mice per group.

(D–F) CNV or ABX mice were infected with influenza virus (PR8-GP33). Mice received 30 µg of poly I:C (ABX+pIC group) or PBS (CNV & ABX group) i.n. at d −1 and 100 µg of poly I:C or PBS i.p. at d3. Weight loss (D) and blood oxygen (E) saturation after infection (representative exp. n = 4–6: † signifies mice below 70% initial weight were sacrificed). Weight loss statistics determined by two-way ANOVA.

(F) Survival curve after influenza virus infection. Survival curve is a combination of two independent experiments. CNV n = 10, ABX n = 8, ABX+pIC n = 12. Survival statistics determined by log rank test. *p < 0.05, **p < 0.01, and ***p < 0.001. Data shown are mean ± SEM.