Figure 5.

Early (4 h) promotional effect of HOCbl on iNOS and eNOS protein expression in liver samples from endotoxemic mice: mice (n = 5) were treated with HOCbl at the low dose protocol (0.2 mg/kg i.p.) at –1 h, +1 h, and +2 h and challenged with LPS (0.1 mg/kg i.p.) at time 0. Liver samples were collected and processed as described in Materials and Methods, for the Western blot analysis of iNOS and eNOS protein expression. Upper panels ((a), (b)) show membranes probed for iNOS, eNOS, with tubulin as the loading control; lower panels show densitometric analysis of the blots. Values are a mean ± SEM of triplicate experiments. *P < 0.05 versus vehicle (PBS-treated) control; ⁺ P < 0.05 versus LPS group.