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mtr-miR159b represents a suitable backbone for amiR expression in M. truncatula. Vertical arrows and numbers indicate the exact positions of degradome tags. The horizontal arrow indicates a loop-to-stem DCL1 processing. The mature miR159b is labeled in red, the miR159b* is labeled in purple. The read numbers of miR159 and miR159* from the small RNA libraries [23] are given.
