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The target sequence, primer, and probe. The 1,154-bp sequence of ribosomal DNA includes an 18S ribosomal RNA gene, a partial sequence (<0–12 bp), internal transcribed spacer 1 (ITS-1, 13–195 bp), the 5.8S ribosomal RNA gene (196–352 bp), internal transcribed spacer 2 (ITS-2, 353–521 bp), 28S ribosomal RNA gene, and a partial sequence (522–>1,154 bp). The target sequence (435–554) includes ITS2 and 28S as well as the partial sequence 28S-ITS2.
