Table 2.
Diagnostic criteria and 28S-ITS2 qPCR results for invasive aspergillosis
|
Diagnostic criteria |
IA category |
Total (%) (n=41) |
||
|---|---|---|---|---|
| Proven (%) (n=4) | Probable (%) (n=18) | Possible (%) (n=19) | ||
|
Host factors |
|
|
|
|
| Neutropenia |
4 (100) |
6 (33.3) |
3 (15.8) |
13 (31.7) |
| T > 38°C with |
|
|
|
|
| Prolonged neutropenia |
4 (100) |
11 (61.1) |
6 (31.6) |
21 (51.2) |
| Immunosuppressant |
1 (25) |
9 (50) |
9 (47.4) |
19 (46.3) |
| Previous IFI |
0 (0) |
4 (22.2) |
3 (15.8) |
7 (17.1) |
| With AIDS |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
| GVHD |
0 (0) |
3 (16.7) |
2 (10.5) |
5 (12.2) |
| Corticosteroids |
3 (75) |
4 (22.2) |
7 (36.8) |
14 (34.1) |
|
Clinical criteria |
|
|
|
|
| Halo sign |
3 (75) |
17 (94.4) |
12 (63.1) |
32 (78) |
| Air-crescent sign |
2 (50) |
1 (5.6) |
2 (10.5) |
5 (12.2) |
| Cavity |
1 (25) |
3 (16.7) |
0 (0) |
4 (9.8) |
| Symptoms of LRI |
3 (75) |
8 (44.4) |
13 (68.4) |
24 (58.5) |
| Permanent fever |
2 (50) |
7 (38.9) |
13 (68.4) |
22 (53.7) |
|
Mycological criteria |
|
|
|
|
| Positive sputum microscopy |
0(0) |
6 (33.3) |
0 (0) |
6 (14.6) |
| Positive sputum culture |
1 (25) |
5 (27.8) |
0 (0) |
6 (14.6) |
| G test positive |
1 (25) |
8 (44.4) |
1 (5.3) |
10 (24.4) |
| No bacterial positive |
0 (0) |
6 (33.3) |
3 (15.8) |
9 (22) |
|
Histology |
|
|
|
|
| Biopsy specimen of the lung |
4 (100) |
0 (0) |
0 (0) |
4 (9.8) |
|
qPCR results (copies/μL) (median, range) |
123.47 |
43.65 |
16.15 |
28.12 |
| (25.96–266.95) | (0–549.5) | (0–98.76) | (0–549.5) | |
qPCR, real-time qualitative polymerase chain reaction; IA, invasive aspergillosis; IFI, invasive fungal infections; AIDS, acquired immune deficiency syndrome; GVHD, graft versus host disease; LRI, lower respiratory infections.