Table 2.
Strains and plasmids used in this study
| Strains or plasmid | Strain and genotype/phenotype | Source |
|---|---|---|
|
Caldicellulosiruptor |
|
|
|
C. bescii DSM6725 |
Wild type / (ura+/5-FOAS) |
DSMZ1 |
|
C.hydrothermalis DSM18901 |
Wild type / (ura+/5-FOAS) |
DSMZ1 |
|
C. kristjansonii DSM12137 |
Wild type / (ura+/5-FOAS) |
DSMZ1 |
|
C. saccharolyticus DSM8903 |
Wild type / (ura+/5-FOAS) |
DSMZ1 |
|
C. obsidiansis ATCC BAA-2073 |
Wild type / (ura+/5-FOAS) |
DSMZ1 |
|
C. lactoaceticus DSM9545 |
Wild type / (ura+/5-FOAS) |
DSMZ1 |
|
C. kronotskyensis DSM12137 |
Wild type / (ura+/5-FOAS) |
DSMZ1 |
| JWCB005 |
C. bescii ΔpyrFA / (ura-/5-FOAR) |
[13] |
| JWCB018 |
C. bescii ΔpyrFAΔcbeI / (ura-/5-FOAR) |
This study |
| JWCH003 |
C. hydrothermalis IScahyI insertion mutation in pryF gene / (ura-/5-FOAR) |
[13,36] |
| JWCH005 |
JWCH003 transformed with M.CbeI methylated pDCW89 / (ura+/5-FOAS) |
[13] |
|
Escherichia coli |
|
|
| JW291 |
DH5α containing pDCW88 (ApramycinR) |
This study |
| JW292 |
DH5α containing pDCW89 (ApramycinR) |
[13] |
| Plasmids |
|
|
| pDCW88 |
cbeI kcock-out vector (ApramycinR) |
This study |
| pDCW89 | E. coli/Caldicellulosiruptor species shuttle vector (ApramycinR) | [13] |
1German collection of microorganisms and cell cultures.