Table 4.
Primers used for the Q-PCR assay
| Gene symbol | Primer sequence (5′ → 3′) | Amplicon length (bp) | GenBank No. |
|---|---|---|---|
|
ACTB* |
F: TCTGGCACCACACCTTCT |
114 |
DQ178122 |
| |
R: TGATCTGGGTCATCTTCTCAC |
|
|
|
TBP* |
F: GATGGACGTTCGGTTTAGG |
124 |
DQ178129 |
| |
R: AGCAGCACAGTACGAGCAA |
|
|
|
TOP2B* |
F: AACTGGATGATGCTAATGATGCT |
137 |
AF222921 |
| |
R: TGGAAAAACTCCGTATCTGTCTC |
|
|
|
ACADL |
F: GTAAGAACAAATGCCAAGA |
103 |
NM_213897 |
| |
R: CAGCCACTACAATCACAAC |
|
|
|
ADIPOQ |
F: GGGTCACTGTCCCTAAC |
209 |
NM_214370 |
| |
R: GTCCTGGTACTGGTCGT |
|
|
|
ADIPOR1 |
F: TGGCTGAAGGACAACGAC |
228 |
NM_001007193 |
| |
R: CAAGAAGAACATCCCAAACAC |
|
|
|
CAV3 |
F: GCATCAGCCATATCTACTCACT |
107 |
NM_001037149 |
| |
R: CACTTCTTTCCGCAGCAT |
|
|
|
MDH1 |
F: TAAGGTTATCGTGGTGGG |
124 |
U44846 |
| |
R: TGCTTTAGCTCGGTTGTG |
|
|
|
MDH2 |
F: CGAGGTGGTCAAGGCTAAG |
172 |
M16427 |
| |
R: CAATGGCGTGGAGAAATAC |
|
|
|
ME 1 |
F: GTTGCCCTTGGTGTTGT |
212 |
X93016 |
| |
R: GGATAAATGGTGGCTGTC |
|
|
|
Insig1 |
F: TCAACCACGCCAGTGCTAA |
251 |
AY336601.2 |
| |
R: CACGCCTCCCGAGAAGAAA |
|
|
|
SCAP |
F: CGCCTGAAACAGAAATCG |
213 |
XM_001924223 |
| R: TCTCCTGAGCCTCCAACA |
*: ACTB (β actin), TBcP (TATA box binding protein) and TOP2B (topoisomerase II β) are the endogenous control genes.