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. Author manuscript; available in PMC: 2013 Jun 12.
Published in final edited form as: Eur J Immunol. 2011 Aug 12;41(9):2677–2687. doi: 10.1002/eji.201041327

Figure 5.

Figure 5

Intracellular CD4+ T-cell cytokine expression and in vitro cytokine cross-regulation. (A) mLNCs from 7 wk-infected unimmunized and SEA/CFA-immunized BL/6 mice were stimulated in vitro with SEA and stained with anti-CD4, anti-IL-17 and anti-IFN-γ mAb as described in the Materials and Methods. Data represent the percentage of CD4+ T cells that express IL-17 and IFN-γ from one experiment representative of two with similar results, using pooled mLNCs from 3 to 5 mice from each group. (B and C) mLNCs from SEA/CFA-immunized BL/6, IFN-γ−/− and IL-17−/− mice were cultured in the absence or presence of SEA plus rIFN-γ or rIL-17 at the indicated concentrations. (B) IL-17 and (C) IFN-γ levels were measured after 48 h of culture by ELISA. Bars represent the mean+SD of triplicate determinations. **p<0.01 are between cultures containing recombinant cytokines and those with SEA alone and were determined by ANOVA followed by Tukey's multiple comparison test. Data are from one experiment representative of three with similar results.