Table 2. Levels of adenine nucleotides, Pi, phosphorylation potential and adenylate energy charge in freeze-clamped liver following perfusion with or without GKA.
ATP, ADP, AMP and Pi were determined in extracts of freeze-clamped liver after perfusion without (Control) or with GKA [(+) GKA] as indicated in the Experimental section. The phosphorylation potential was calculated from the measured total concentrations of ATP, ADP and Pi as: [ATP]/[ADP]×[Pi ]. The adenylate energy charge was calculated as: ([ATP] + [ADP]/2)/([ATP] + [ADP] + [AMP]). Results are means ± S.D. of three livers per group. P values were determined by Student’s t test using Prism 5 software.
| Control (μmol per g of wet mass) | (+) GKA (μmol per g of wet mass) | P value | |
|---|---|---|---|
| ATP | 3.4 ± 0.1 | 4.1 ± 0.4 | 0.03 |
| ADP | 1.4 ± 0.2 | 1.1 ± 0.4 | N.S. |
| AMP | 0.7 ± 0.1 | 0.5 ± 0.1 | 0.08 |
| Pi | 4.8 ± 0.5 | 3.9 ± 0.6 | 0.07 |
| Phosphorylation potential | 0.52 ± 0.14 | 1.05 ± 0.32 | 0.03 |
| Adenylate energy charge | 0.74 ± 0.01 | 0.82 ± 0.03 | 0.06 |
N.S., not significant.