. Author manuscript; available in PMC: 2014 Jun 1.

Published in final edited form as: Protein Expr Purif. 2013 Apr 3;89(2):203–209. doi: 10.1016/j.pep.2013.03.013

Table 3.

Summary of the purification of MBP-4A-S1R

	Volume	Protein concentration	Total protein	Total binding activity	Specific binding^b	Fold purification
	mL	mg/mL	mg^a	cpm x 10⁹	(cpm/mg) x 10⁶
Lysate^a	330	9.4	3102	4.4	1.4	1
Membrane fraction	75	9.7	728	5.6	7.7	5
Amylose affinity	43	0.6	26	5.8	225	158
Gel filtration	1	13.7	14	3.4	248	175^c

Prepared from 4 L of E. coli B834-pRARE2 culture, 23.3 g wet cells.

Normalized to mg of total protein instead of nmol of MBP-4A-S1R.

The 175-fold purification of MBP-4A-S1R was calculated based on increase in specific binding activity for the ligand [H³]-(+)-pentazocine.