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Western blot analysis of AtMHX under reducing and non-reducing conditions. Samples of tobacco plants overexpressing AtMHX were treated under reducing (β-mercaptoethanol) or non-reducing (NEM) conditions as detailed in the Methods. The size of the reduced and non-reduced forms (53.7 and 48.8 kDa, respectively) of AtMHX was determined based on size markers. NT – a sample of non-transformed tobacco plants treated under reducing conditions.
