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. 2013 Jun 6;10:21. doi: 10.1186/1743-8977-10-21

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Copyright © 2013 Campagnolo et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Macroscopic and microscopic analysis of placentas and embryos after maternal exposure to PEG-SWCNTs. A. A Fetus showing abnormal development (left) is compared to one from a control female (right). B. The placenta nourishing the abnormal fetus (left) appeared abnormally vascularised and slightly smaller than the one from the control fetus (right). C-F. Reduced vascularisation in abnormal placentas (C) compared to normal ones (D) was demonstrated in Azan-Mallory stained sections; similar results were obtained by immunostaining, using the anti-CD31 antibody: (E) placentas nourishing abnormal fetuses displayed reduced CD31 expression, compared to normal tissue (F).