Skip to main content
. 2013 May 6;6:69. doi: 10.1186/1754-6834-6-69

Table 1.

Plasmids constructed and used in this study

Plasmid Relevant characteristicsa, b Reference
pFQ9R
Apr Sper, pKW1188sl derivative containing Omega, Trbc terminator, Prbc promoter
[5]
pXT37b
Apr, Sper, pUC9' derivative containing upstream and downstream fragments of slr1609, Omega and PpetE promoter.
[5]
pXT119
Apr, pMD18-T derivative containing upstream and downstream fragments of slr1556
This study
pLX1
Apr Sper, pFQ9R derivative containing sll0208 gene, Prbc promoter
This study
pLX3
Apr Sper, pFQ9R derivative containing sll0208 and sll0209 gene, Prbc promoter
This study
pLX4
Apr Sper, pXT37b derivative containing sll0209 gene, PpetE promoter
This study
pLX5
Apr Sper, pFQ9R derivative containing orf1593 and orf1594 gene, Prbc promoter
This study
pLX6
Apr Sper, pFQ9R derivative containing npun1710 and npun 1711 gene, Prbc promoter
This study
pLX9
Apr Sper, pFQ9R derivative containing orf1594 and npun 1711 gene, Prbc promoter
This study
pLX13
Apr, Kanr, pXT119 derivative containing sll0208 and sll0209 gene, CK2, and Prbc promoter.
This study
pLX14
Apr, pGEM-T Easy derivative containing sll0208 and sll0209 gene, Prbc promoter.
This study
pLX27
Apr, pGEM-T Easy derivative containing sll0208 and sll0209 gene, Prbc promoter, Trbc terminator.
This study
pLX28
Apr, Kanr, pGEM-T Easy derivative containing sll0208 and sll0209 gene, Prbc promoter, Trbc terminator, CK2.
This study
pLX59 Apr Sper, pFQ9R derivative containing two fragments of Prbc promoter , sll0208 and sll0209 gene. This study

a Ap, Ampicillin. Spe, Spectinomycin. Kan, kanamycin.

bCK2 for kanamycin antibiotic resistance gene and Omega for spectinomycin antibiotic resistance gene.