Table 1.
Nucleotide sequences of primers and probes used in this study
Name | Sequence (5′ to 3′) | Reference |
---|---|---|
AdV2F |
CCA GGA CGC CTC GGA GTA |
[18] |
AdV2R |
AAA CTT GTT ATT CAG GCT GAA GTA CGT |
[18] |
AdV2pr |
FAM- AGT TTG CCC GCG CCA CCA CCG – BHQ1* |
[18] |
AdV4F |
GGA CAG GAC GCT TCG GAG TA |
[18] |
AdV4R |
CTT GTT CCC CAG ACT GAA GTA GGT |
[18] |
AdV4pr |
FAM- CAG TTC GCC CGY GCM ACA G – BHQ1* |
[18] |
FGFP |
TGA TAC CCT TGT TAA TAG A |
This study |
RGFP |
ATT GTG TGA GTTATA GTT G |
This study |
GFPpr1 |
GGT ATT GAT TTT AAA GAA GAT GG – FAM** |
This study |
GFPpr2 |
LC705 – CAT TCT TGG GCA CAA ATT GGA- Ph** |
This study |
AD1SEQ |
CTG ATG TAC TAC AAC AGC ACT GGC AAC ATG GG |
[32] |
AD2SEQ |
GCG TTG CGG TGG TGG TTA AAT GGG TTT ACG TTG TCC AT |
[32] |
F14MUT |
TCT GCG GGT AAT TTA CTA ACT AG |
This study |
R14MUT | ATC TCC TGT GTT CCA GGA CCA | This study |
* The hydrolysis probes were labeled with 6-carboxyfluorescein (FAM) at the 5’end; however, the 3’end was labeled with a Black Hole Quencher 1 (BHQ1) instead of the carboxytetramethyl-rhodamine (TAMRA) quencher previously described [18].
** Abbreviations for the hybridization probes targeting the internal control (pGFP) are as follows: [FAM], 6-carboxyfluorescein; [LC705], LightCycler-Red 705; Ph, 3′-phosphate.