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. 2013 Jun 7;10:184. doi: 10.1186/1743-422X-10-184

Table 1.

Nucleotide sequences of primers and probes used in this study

Name Sequence (5′ to 3′) Reference
AdV2F
CCA GGA CGC CTC GGA GTA
[18]
AdV2R
AAA CTT GTT ATT CAG GCT GAA GTA CGT
[18]
AdV2pr
FAM- AGT TTG CCC GCG CCA CCA CCG – BHQ1*
[18]
AdV4F
GGA CAG GAC GCT TCG GAG TA
[18]
AdV4R
CTT GTT CCC CAG ACT GAA GTA GGT
[18]
AdV4pr
FAM- CAG TTC GCC CGY GCM ACA G – BHQ1*
[18]
FGFP
TGA TAC CCT TGT TAA TAG A
This study
RGFP
ATT GTG TGA GTTATA GTT G
This study
GFPpr1
GGT ATT GAT TTT AAA GAA GAT GG – FAM**
This study
GFPpr2
LC705 – CAT TCT TGG GCA CAA ATT GGA- Ph**
This study
AD1SEQ
CTG ATG TAC TAC AAC AGC ACT GGC AAC ATG GG
[32]
AD2SEQ
GCG TTG CGG TGG TGG TTA AAT GGG TTT ACG TTG TCC AT
[32]
F14MUT
TCT GCG GGT AAT TTA CTA ACT AG
This study
R14MUT ATC TCC TGT GTT CCA GGA CCA This study

* The hydrolysis probes were labeled with 6-carboxyfluorescein (FAM) at the 5’end; however, the 3’end was labeled with a Black Hole Quencher 1 (BHQ1) instead of the carboxytetramethyl-rhodamine (TAMRA) quencher previously described [18].

** Abbreviations for the hybridization probes targeting the internal control (pGFP) are as follows: [FAM], 6-carboxyfluorescein; [LC705], LightCycler-Red 705; Ph, 3′-phosphate.