Table 3.
Summary of the method performance characteristics compared to the modified gold standard*
| Method | Sensitivity (%; 99% CI) | Specificity (%; 99% CI) | Accuracy (%; 99% CI) | Precision (%; 99% CI) | Kappa (99% CI) |
|---|---|---|---|---|---|
| Virus culture |
69.2 (56.0-69.2) |
100 (96.7-100) |
93.9 (88.6-93.9) |
100 (80.8-100) |
0.783 (0.596-0.783) |
| Nucleic acid extraction and real-time PCR |
97.4 (86.4-97.4) |
100 (97.3-100) |
99.5 (95.1-99.5) |
100 (88.6-100) |
0.984 (0.844-0.984) |
| Homogenization with heat treatment and real-time PCR | 100 (89.7-100) | 100 (97.4-100) | 100 (95.6-100) | 100 (89.7-100) | 1.000 (0.871-1.000) |
* A case was defined by concordant results (positive or negative) between at least two assays. A manual nucleic acid extraction and commercial real-time PCR was used to resolve discrepant results. TP true positive, TN true negative, FP false positive, FN false negative, CI confidence interval.