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. 2013 May 24;10:67. doi: 10.1186/1742-2094-10-67

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Copyright © 2013 Cravens et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of immune cell subsets in the central nervous system (CNS) post immunization with complete Freund adjuvant (CFA)/myelin oligodendrocyte glycoprotein (MOG)_p35-55. CNS mononuclear cells from unimmunized (gray bars) neonates (A, C, E) and adults (B, D, F) and from neonates and adults that had been immunized with CFA/MOG_p35-55 10 days earlier (black bars). Flow cytometry was used to examine the number of CD45⁺ immune cells, granulocytes and microglia/g of CNS (A, B), lymphocyte subsets (C, D), and myeloid subsets in panels (E, F). Figures are representative of data obtained from at least three independent experiments.