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Quantitative RT-PCR analysis of PGRN mRNA levels. PBMCs were separated by Red Blood Cell Lysis Buffer (Pharmacia Diagnostics, Uppsala, Sweden), and total RNA was isolated by Trizol Reagent (Invitrogen, America) according to the manufacturer’s instructions. Each sample was run in triplicate. Expression of mRNAs was normalized to the expression of GAPDH gene.
