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. 2013 May 25;12:53. doi: 10.1186/1475-2859-12-53

Table 2.

Oligonucleotides used in this work

Name Sequence Description
4125
5′-tgtcatcactgctccatctt-3′
S. cerevisiae TDH1 gene
4126
5′-ttaagccttggcaacatatt-3′
S. cerevisiae TDH1 gene
5092
5′-gcgatctcgaggtcctagaatatgtatactaatttgc-3′
C. albicans PGK1 ORF (acc. U25180)
5091
5′-cgcgaattcccatggttagtttttgttggaaagagcaac-3′
C. albicans PGK1 ORF (acc. U25180)
5423
5′-gcgatctcgagaaagaaacgacccatccaagtgatg-3′
CsPGK1 promoter −1500
5439
5′-tggactagtacatgcatgcggtgagaaagtagaaagcaaacattgtatatagtcttttctattattag-3′
CsPGK1 promoter-MEL5 fusion
5441
5′-gcgatctcgagaaaatgttattataacactacac-3′
CsTDH1 promoter −600
5440
5′-tggactagtacatgcatgcggtgagaaagtagaaagcaaacattttgtttgatttgtttgttttgtttttgtttg-3′
CsTDH1 promoter MEL5 fusion
5427
5′-acttggccatggtatatagtcttttctattattag-3′
CsPGK1 promoter –LhLDH fusion
LhLDH1
5′-atggcaagagaggaaaaacctcgtaaag-3′
LhLDH probe (fwd)
LhLDH2
5′-ccacgaagagtcattgacgaaccttaa-3′
LhLDH probe (rev)
BmLDH1
5′-ccaacaaaaccagttccgataacg-3′
BmLDH probe (fwd)
ScerGal10t
5′-ccggactagttggtacagagaacttgtaaacaattcgg-3′
BmLDH probe (rev)
RoLDHA1
5′-ctagctcagaacaatggtattacactcaaaggtcgccatcg-3′
RoLDH probe (fwd)
RoLDHA2
5′-cgcggatccgaattctcaacagctacttttagaaaaggaag-3′
RoLDH probe (rev)
5116
5′-ccggaattcgatatctgggcwggkaatgccaaygarttraatgc-3′
PDC1 and PDC2 probes (fwd)
5118
5′-cgcggattcaggcctcagtangaraawgaaccngtrttraartc-3′
PDC1 and PDC2 probes (rev)
G418-5′
5′-ctagtctagaacaatgagccatattcaacgggaaacg-3′
G418R probe (fwd)
G418-3′
5′-cgcggatccgaattcttagaaaaactcatcgagcatcaaatg-3′
G418R probe (rev)
Cs1
5′-ctagtctagatttgtttgatttgtttgttttgtttttgtttg-3′
C. sonorensis TDH1 promoter
Cs2
5′-ctagtctagatgtatatagtcttttctattattag-3′
C. sonorensis PGK1 promoter
Cs5
5′-ggcccgcggccgctacaagtgattcattcattcact-3′
C. sonorensis PDC1 5′ flank
Cs6
5′-ccctgggcccctcgaggatgatttagcaagaataaattaaaatgg-3′
C. sonorensis PDC1 5′ flank
Cs7
5′-gggactagtggatccttgaagtgagtcagccataaggacttaaattcacc-3′
C. sonorensis PDC1 3′ flank
Cs8
5′-aaggccttgtcgacgcggccgcttggttagaaaaggttgtgccaatttagcc-3′
C. sonorensis PDC1 3′ flank
Cs26
5′-gggacgggcccgcggccgcttacagcagcaaacaagtgatgcc-3′
C. sonorensis PDC2 5′ flank
Cs27
5′-ccctgggcccctcgagtttgatttatttgctttgtaaagagaa-3′
C. sonorensis PDC2 5′ flank
Cs29
5′-tggactagttagatagcaattcttacttgaaaaattaattgaagcattacc-3′
C. sonorensis PDC2 3′ flank
Cs30 5′-ggcccgcggccgctaaatataattatcgcttagttattaaaatgg-3′ C. sonorensis PDC2 3′ flank