Table 4.
C. sonorensis ATCC32109 derived strains constructed and studied in this work
| Description | Transformed with plasmid(s) |
|---|---|
|
pdc1∆ |
pMI267 |
|
pdc2∆ |
pMI287 |
|
pdc1∆ pdc2∆ |
pMI267, pMI287 |
|
pdc1∆::BmLDH |
pMI265 |
|
pdc2∆::BmLDH |
pMI286 |
|
pdc1∆::BmLDH pdc2∆ |
pMI265, pMI287 |
|
x::BmLDH |
pMI265 |
|
x::BmLDH y::BmLDH |
pMI265 |
|
x::LhLDH |
pMI246 |
|
x::LhLDH |
pMI247 |
|
x::LhLDH y::LhLDH |
pMI257 |
|
x::LhLDH-LhLDH-LhLDH |
pMI247 |
|
pdc1∆::LhLDH |
pMI257 |
|
pdc1∆::RoLDH |
pMI266 |
|
pdc1∆::LhLDH pdc2∆ |
pMI257, pMI287 |
|
pdc1∆::RoLDH pdc2∆ |
pMI266, pMI287 |
|
pdc1∆::LhLDH pdc2∆::LhLDH |
pMI257, pMI288 |
| pdc1∆::BmLDH pdc2∆::BmLDH | pMI265, pMI286 |
The heterologous LhLDH, BmLDH, and RoLDH genes were expressed under the control of the C. sonorensis PGK1 promoter; the MEL5 and G418R marker genes were expressed under the C. sonorensis GPD1 or PGK1 promoter (not indicated in the table). x:: and y:: indicate that the site of integration is not known. Two consecutive transformations were made to construct a strain where two plasmids are listed.