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. Author manuscript; available in PMC: 2013 Jun 12.
Published in final edited form as: J Health Care Poor Underserved. 2013 Feb;24(1 0):93–103. doi: 10.1353/hpu.2013.0044

Figure 1.

Figure 1

A) Metformin causes concentration dependent apoptosis and proliferative arrest in MDa-MB-468 breast cancer cells. 1 × 105 MDA-MB-468 cells were exposed to metformin for 24 and 48 hours at indicated concentration, and images captured using phase contrast microscopy (400X). White arrows indicate early senescent cells. Black arrow indicate plasma membrane blebbing, indicative of apoptosis. B, C ) 1 mM metformin is the minimum effective concentra-tion for inhibition of cell viability in MDa-MB-468 cells. Cells were plated at 1 × 105 cells per well and after 24 hours, incubated with increasing concentrations of metformin for 72 hours, quantified and normalized to % control (untreated). In panel C, cells were treated for 48 hours, and cell viability determined at day 7 as described. Error bars represent standard deviation of the mean Statistical analysis ANOVA with post-hoc analysis A) or Student’s T-test B).* denotes where p≤0.05 when compared to control.