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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: Bioconjug Chem. 2013 Apr 24;24(5):803–810. doi: 10.1021/bc300613h

Figure 5.

Figure 5

Differential fluorescence enhancement of membranes prepared with EAK16-II mixed with dL5 or dL5_EAK. (a) Samples were prepared by mixing the respective FAP proteins with EAK16-II with MG in PBS+ containing Congo red. (b) Excitation spectra (emission = 680 nm +/− 10 nm) and (c) emission spectra (excitation = 480 nm +/− 10 nm) of dL5_EAK membrane and dL5 mixed with EAK16-II. (d) Average fluorescence intensity (excitation = 635 nm; emission = 680 nm) of re-suspended membrane pellets of EAK 16-II prepared with dL5_EAK or dL5 at the indicated molar ratios (FAP to EAK16-II). No fluorescence above unbound fluorogen background was detected for MG with EAK16-II alone (data not shown).