Figure 9. CCL17 and CCL22 are induced in autologous tumor-T_FH co-cultures.

T_FH (CD3⁺CD4⁺CD25⁻PD1^hiCXCR5^hi) were sorted by FACS from three cryopreserved FL biopsy samples and three tonsil samples. They were cultured in the absence or presence of autologous tumor cells or tonsil B cells (0.35×10⁶ tumor cells/well). After 96 h, CCL17 (A and C) and CCL22 (B and D) production was assessed in the culture supernatants by ELISA. All P values were calculated using paired Student’s t test.