Table 3.

CSF proteins showing at least a two-fold difference in expression between RLS/WED and control subjects

Identified protein	Gi access no.	Gene name	Function	Fold change	MW	pI
Proteins that are down-regulated-regulated in RLS/WED CSF
Apolipo protein A-I (precursor)	4557321	APOA1	Major protein component of high density lipoprotein (HDL) in plasma. Promotes cholesterol efflux from tissues to the liver for excretion.	−1.97	30758.93	5.56
Alpha-1-acid glycoprotein 1	112877	AGP	Reduces neutrophil phagocytosis of bacteria, inhibits neutrophil migration and superoxide production, reduces activated platelet aggregation, and inhibits activated lymphocytes proliferation.	−5.25	23724.84	4.93
Proteins that are up-regulated in RLS/WED CSF
vitamin D-binding protein (precursor)	181482	GC	Binds vitamin D and its metabolites and transports them to target tissues	2.32	54611.68	5.4
Prostaglandin D2 synthase 21kDa (brain)	55962673	PTGDS	Neuromodulator and trophic factor in the CNS. Involved in smooth muscle contraction and relaxation; and inhibits platelet aggregation.	2.18	23049.57	9.92
cystatin C	181387	CST3	Inhibitor of lysosomal proteinases and an extracellular inhibitor of cysteine proteases	11.32	16047.18	9
β-Hemoglobin	183830	HBB	most common form of hemoglobin in adult humans	4.53	16086.3	6.75