Table 1.

Relative gene expression in the engineered strains growing anaerobically on LB-CB medium supplemented with 12.5 mM fumarate

Strains	caiT	caiB	caiC	fixA
BW ∆caiA	1.443 ± 0.20	0.86 ± 0.21	0.81 ± 0.06	1.32 ± 0.19
BW ∆aceK p37cai	3.00 ± 0.16	3.08 ± 0.24	2.18 ± 0.20-	1.95 ± 0.17
BW ∆aceK ∆caiA p37cai	3.23 ± 0.17	1.76 ± 0.10	1.47 ± 0.12	1.84 ± 0.10

For each gene, the transcription level of that gene in the wild type strain was used as reference to normalize the data. Relative gene expression in the wild type strain is, therefore, taken as 1. The results are the averages of three independent measurements of each gene/condition in three independent experiments.