Table 2.
Strain and conditions | qL-car | qSuc | -qFum | qAcet | qEtOH | qForm |
---|---|---|---|---|---|---|
Anaerobic cultures | ||||||
LB-CB | ||||||
BW25113 |
9.30 ± 0.12 |
8.43 ± 0.22 |
--- |
15.71 ± 0.28 |
7.23 ± 0.20 |
4.21 ± 0.14 |
BW ∆aceK ∆caiA p37cai |
18.87 ± 0.23*** |
7.20 ± 0.17*** |
--- |
17.44 ± 0.16*** |
2.49 ± 0.12*** |
12.96 ± 0.42*** |
LB-CB + fumarate (12.5 mM) | ||||||
BW25113 |
14.23 ± 0.15 |
13.89 ± 0.54 |
17.74 ± 0.26 |
13.15 ± 0.36 |
4.8 ± 0.32 |
N.D. |
BW ∆aceK ∆caiA p37cai |
27.75 ± 0.17*** |
10.31 ± 0.32*** |
19.51 ± 0.41*** |
16.44 ± 0.21*** |
3.95 ± 0.27*** |
N.D. |
Aerobic cultures | ||||||
LB-CB | ||||||
BW25113 |
--- |
N.D. |
--- |
9.49 ± 0.23 |
0.43 ± 0.05 |
--- |
BW ∆aceK ∆caiA p37cai |
6.15 ± 0.10*** |
N.D. |
--- |
7.79 ± 0.12*** |
1.17 ± 0.08* |
--- |
LB-CB + fumarate (12.5 mM) | ||||||
BW25113 |
--- |
3.32 ± 0.11 |
9.42 ± 0.24 |
5.74 ± 0.10 |
0.15 ± 0.02 |
--- |
BW ∆aceK ∆caiA p37cai | 7.02 ± 0.07*** | 2.95 ± 0.08 | 6.82 ± 0.17*** | 8.31 ± 0.14*** | 1.26 ± 0.06*** | --- |
Specific production/consumption rates of L(−)-carnitine and the main extracellular metabolites were calculated during the early exponential phase of cultures. All rates are expressed in mmol g-1 h-1. Pairwise statistical comparison of the parameters assessed for both strains was perfomed with ANOVA and Tukey tests. Adjusted p-values are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001.