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. 2013 May 29;12:56. doi: 10.1186/1475-2859-12-56

Table 2.

Metabolic performance of wild-type (BW25113) and engineered (BW ΔaceK ΔcaiA p37 cai ) E. coli strains during L(−)-carnitine production in anaerobic and aerobic conditions

Strain and conditions qL-car qSuc -qFum qAcet qEtOH qForm
Anaerobic cultures
LB-CB
  BW25113
9.30 ± 0.12
8.43 ± 0.22
---
15.71 ± 0.28
7.23 ± 0.20
4.21 ± 0.14
  BW ∆aceKcaiA p37cai
18.87 ± 0.23***
7.20 ± 0.17***
---
17.44 ± 0.16***
2.49 ± 0.12***
12.96 ± 0.42***
LB-CB + fumarate (12.5 mM)
  BW25113
14.23 ± 0.15
13.89 ± 0.54
17.74 ± 0.26
13.15 ± 0.36
4.8 ± 0.32
N.D.
  BW ∆aceKcaiA p37cai
27.75 ± 0.17***
10.31 ± 0.32***
19.51 ± 0.41***
16.44 ± 0.21***
3.95 ± 0.27***
N.D.
Aerobic cultures
LB-CB
  BW25113
---
N.D.
---
9.49 ± 0.23
0.43 ± 0.05
---
  BW ∆aceKcaiA p37cai
6.15 ± 0.10***
N.D.
---
7.79 ± 0.12***
1.17 ± 0.08*
---
LB-CB + fumarate (12.5 mM)
  BW25113
---
3.32 ± 0.11
9.42 ± 0.24
5.74 ± 0.10
0.15 ± 0.02
---
BW ∆aceKcaiA p37cai 7.02 ± 0.07*** 2.95 ± 0.08 6.82 ± 0.17*** 8.31 ± 0.14*** 1.26 ± 0.06*** ---

Specific production/consumption rates of L(−)-carnitine and the main extracellular metabolites were calculated during the early exponential phase of cultures. All rates are expressed in mmol g-1 h-1. Pairwise statistical comparison of the parameters assessed for both strains was perfomed with ANOVA and Tukey tests. Adjusted p-values are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001.