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SDS-and native PAGE of eYFP and sfYFP expressed in P.putida KT2440 and E.coli Bl21. Coomassie-stained SDS-PAGE (top), native-PAGE (mid) and native-PAGE fluorescence scan (bottom) of affinity purified 1) eYFP (pTD-C_eYFPTwinStrep); 2) sfYFP (pTD-C_sfYFPTwinStrep) in E.coli BL21; 3) eYFP (pTD-C_eYFPTwinStrep) and 4) sfYFP (pTD-C_sfYFPTwinStrep) in P.putida KT2440. M = PageRuler Unstained Broad Range Protein Ladder (ThermoScientific) with 70, 50, 40, 30, 20 kDa bands.
