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Fluorescence microscopy images of bacteria expressing export optimized super folder YFP. A) sfYFP expressed in the cytoplasm (left) and targeted to the periplasm (right) in E.coli BL21 and B) sfYFP expressed in the cytoplasm (left) and targeted to the periplasm (right) in P.putida KT2440 from plasmids pTD-C_sfYFPTwinStrep and pTDpelB-C_sfYFPTwinStrep (scale bar 2.5 μm).
