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. Author manuscript; available in PMC: 2013 Jun 12.
Published in final edited form as: Mol Cancer Ther. 2009 Oct;8(10):2959–2968. doi: 10.1158/1535-7163.MCT-09-0292

Figure 2.

Figure 2

(A) 3′RACE analysis of ABCG2 mRNA in the S1M1 series of resistant cell lines. The schematics for the 3′RACE assay can be found in Supplementary Figure 1. The first PCR and the nested PCR 1 gave the same pattern of PCR products. Only the nested PCR 1 is shown. In miR-519c inhibitor-transfected S1 parental cells, a long PCR product (about 2 kb, long arrow) and three much smaller fragments (~200, 300 and 400 bp, shorter arrows) were obtained. In all resistant S1M1 sublines, only the smaller fragments were seen. Nested PCR 2 was performed to further confirm the existence of the long ABCG2 3′UTR fragment in the parental S1 cells only. (B) FISH analysis of chromosome metaphase spreads with whole chromosome paints. Chromosome 4 is painted with WCP 4 Spectrum Orange and chromosome 17 is painted with WCP 17 Spectrum Green; DAPI counterstain. S1 parent line: two normal chromosomes 4; S1M1 0.4, S1M1 0.8, S1M1 1.6, S1M1 3.2, S1M1 80: one normal chromosome 4, one normal 17, and t(4;17)(q21-q22; p13). Earlier studies had shown that the ABCG2 signal is split at the breakpoint (31).