Table 1.

Reproducibility of the microarray for autoantibody-antigen assay

	a Intra-array coefficient of variation		b Inter-array coefficient of variation
	IgG-complex	IgM-complex	IgG-complex	IgM-complex
CV<0.1	86.1%	80.0%	59.5%	50.3%
0.1<CV<0.2	9.7%	12.3%	31.1%	31.9%
0.2<CV<0.3	1.3%	4.6%	4.5%	8.1%
0.3<CV	2.9%	3.2%	5.0%	7.3%

Five microarrays were independently incubated with the same colon cancer plasma. Median spot intensity at each antibody position was used and only spots showing >3000 intensity were selected for CV calculation.

^a $\text{Intra}-\text{array}\text{CV}=\frac{\alpha \text{Variation}\text{of}\text{triplicate}\text{spot}\text{intensities}}{\mu \text{Average}\text{of}\text{triplicate}\text{spot}\text{intensities}}$

^b $\text{Inter}-\text{array}\text{CV}=\frac{\sigma \text{Variation}\text{of}5-\text{array}\text{median}\text{spots}\text{representing}\text{the}\text{same}\text{triplicate}}{\mu \text{Average}\text{of}5-\text{array}\text{median}\text{spots}\text{representing}\text{the}\text{same}\text{triplicate}}$