Fig. 2.

Differential effects of APAP or AMAP on protein nitration. Hepatic mitochondrial (A) and cytosolic (B) proteins from mice exposed to the indicated agents for 2 h were subjected to immunoblot analysis with the specific anti-3-NT (upper panels), while specific anti-ATP synthase and actin were used to detect equal protein loading respectively (lower panels). Silver stained images of the affinity-purified nitrated proteins from mitochondria (C) and cytosol (D) are shown (n=4-6/group). Similar results to those shown in the Figures (A-D) were observed by at least two separate experiments.