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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2013 Feb 27;60:211–222. doi: 10.1016/j.freeradbiomed.2013.02.018

Fig. 6.

Fig. 6

Reversible changes of nitration and activity of ATP synthase. (A) The levels of mitochondrial ATP synthase in APAP-exposed mice ± NAC for 2 or 24 h were determined by immunoblot analysis. (B) Mitochondrial proteins from the indicated groups were immunoprecipitated using the specific anti-ATP synthase antibody. The immunoprecipitated proteins were then subjected to immunoblot analysis with anti-3-NT antibody (upper panel) or anti-ATP synthase antibody (bottom). (C and D) Mitochondrial ATP synthase activity and levels were determined for the indicated samples, respectively. #, significantly different (*p<0.05) from other groups (n=4-6/group).