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. Author manuscript; available in PMC: 2013 Jun 12.
Published in final edited form as: Biochem J. 2010 Jun 15;428(3):325–346. doi: 10.1042/BJ20100046

TABLE 1.

Cleavage Sequence Specificities1,2

Protease3 Common Aliases Gene Symbol P4 P3 P2 P1 ↓ P1' P2' P3' P4' Protease Source Substrates Ref
GPI Anchored
Prostasin PRSS8, CAP1 PRSS8 R K R K I S G K cDNA ENAC(SCNN1γ) [141]
R/K H/K/R Y/W/R R/K X X X A/S SPD Combinatorial peptide library. SPD [85]
Testisin PRSS21, TESP, TEST1, ESP-1, tryptase 4 PRSS21 Boc F S R MCA SPD 4 peptide screen [18]
Type I
Tryptase γ1 TMT/TPSG1, PRSS31 TPSG1 K W/Y/F R AMC ECD Peptide library [217]
Type II
HAT TMPRSS11D TMPRSS11D N S G R A V T G SPD uPAR [96]
T Y S R S R Y L SPD uPAR [96]
K Q T R G L F G cDNA H3N2 HA protein, MDCK cells [162]
Boc F S R MCA Purified human peptide [54]
DESC1 TMPRSS11E TMPRSS11E R R/Q A/R R V V G G ECD 18 peptide screen [87]
V S A R M A P E hECD, mSPD hPAI-1 & RCL mutant [86,87]
F T F R S A R L mSPD hPCI & RCL mutant [86]
HATL1 TMPRSS11A, DESC3 TMPRSS11A S L L R S T S Q soluble protein SARS S glycoprotein (triSpike) [167]
P T K R S F I E [167]
HATL3 Neurobin, TMPRSS11C Tmprss11c V G R pNA SPD 9 peptide screen [102]
R G H K V A G G cDNA proHATL3 [102]
N T Y R S R K Y mSPD FGF-2 fragment [102]
F F L R I H P D mSPD FGF-2 fragment [102]
C A N R Y L A M mSPD FGF-2 fragment [102]
V R E K S D P H mSPD FGF-2 fragment [102]
P H I K L Q L Q mSPD FGF-2 fragment [102]
Hepsin TMPRSS1 HPN K/P K/Q T/N/L R ECD Peptide library [83]
S Q L R L Q G S ECD Laminin-332 β3 [198]
K Q L R V V N ECD proHGF [121]
P Q G R I V G G cDNA, ECD FVII [83,91]
P V D R I V G G cDNA m proHepsin [101]
P R F K I I G G ECD, cDNA pro-uPA [93]
TMPRSS2 epitheliasin TMPRSS2 R Q S R I V G G cDNA proTMPRSS2 [37]
R Q S R F V L G cDNA HMPV F protein Vero cells [165]
I Q S R G L F G cDNA 1918 HA (South Carolina) 293T cells [164]
K Q T R G L F G cDNA H3N2 HA protein MDCK cells [162]
S K G R S L I G purified PAR2 [112]
Cbz G G R AMC purified Peptide assay [112]
TMPRSS4 MT-SP2, CAP2 TMPRSS4 I Q S R G L F G cDNA 1918 HA (South Carolina) 293T cells [164]
R K R R E A G S cRNA r γENAC xenopus oocytes [145]
L N Y K T N S E cRNA r αENAC xenopus oocytes [145]
F N Y R T I E E cRNA r βENAC xenopus oocytes [145]
L N Q R S I M E cRNA r γENAC xenopus oocytes [145]
Boc Q A R AMC ECD Peptide assay [187]
TMPRSS13 MSPL TMPRSS13 Boc Q/L R/K R MCA 19 peptide screen [84]
R/K K K R G L F G ECD HA-based peptide [161]
TMPRSS5 Spinesin TMPRSS5 Boc Q A R MCA SPD 10 peptide screen [220]
Enteropeptidase PRSS7, Enterokinase PRSS7 D D D K I V G G purified porcine, bSPD Trypsinogen 1 [221,222]
D/A D D K/R I V G Purified bovine, porcine 20 peptide screen [223]
Matriptase MT-SP1, CAP3, TADG-15, PRSS14, ST14, SNC19, epithin (mouse) ST14 R Q AGL R V V G G SPD 18 peptide screen [87]
R/K X S R A SPD Combinatorial peptide library [81]
X R/K S R A SPD Combinatorial peptide library [81]
R Q A R V V G G proMatriptase [69]
P/I Q A/P R I T G G KO, SPD proProstasin (h/m) [99,123]
P R F K I I G G SPD Pro-uPA [81]
S K G R S L I G SPD hPAR2 xenopus oocytes [81]
K Q L R V V N G proHGF [95,224,225]
S K L R V V G G MSP-1 [117]
K Q S R K F V P SPD ECD TRASK [226]
R K G K A G A A ECD, siRNA IGFBP-rP1 [227]
R R V R K E D E VEGFR2 [125]
Matriptase-2 TMPRSS6 TMPRSS6 boc Q A/G R AMC GST-SPD 5 peptide screen [104]
R R A R A/V V G G ECD 18 peptide screen [87]
Matriptase-3 TMPRSS7 TMPRSS7 A A p R pNA mSPD 7 peptide screen [203]
F V R pNA mSPD 7 peptide screen [203]
V S A R M A P E mSPD hPAI-1 & RCL mutant [203]
Polyserase-1/Serase-1B TMPRSS9 TMPRSS9 Boc Q G R AMC SPD 4 peptide screen [22]
Boc Q/L S/T/A R MCA ECD 14 peptide screen [23]
P R F K I I G G ECD Pro-uPA [23]
Corin LRP4, ATC2, TMPRSS10 CORIN Y A P R S L R R cDNA, ECD proANP, HEK293 cells [127,128]
R A P R S P K M ECD proBNP [79]
pyro E F K pNA 5 peptide screen [228]
1

Basic amino acids are highlighted in blue, acidic amino acids in red, and aromatic amino acids in green.

2

Abbreviations: AMC, 7-amino 4-methyl coumarin; Boc, t-butoxycarbonyl; ECD, extracellular domain; GST, Glutathione-S-Transferase; h, human; m, mouse; MCA, 4-methylcoumaryl-7-amide; pNA, p-nitroanilide; r, rat; RCL, reactive center loop; SPD, serine protease domain;

3

Substrate cleavage specificities for DESC4/HATL2, HATL4/TMPRSS11F, HATL5/TMPRSS11B, TMPRSS3, are not known.