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. 2013 Jun 12;8(6):e66224. doi: 10.1371/journal.pone.0066224

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© 2013 Khan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A). Pf-WRS (AAD) expression in parasites (Par) and detection of recombinant Pf-WRS (Rec) by western blot analysis. A larger size protein band was observed (∼73 kDa) in the parasite lysate consistent with gene annotation for (Pf-WRS, residues 1–632). The recombinant Pf-WRS AAD migrated at ∼46.8 kDa as expected from the truncated DNA construct (Pf-WRS AAD, residues 228–632) (B). Localization of Pf-WRS in asexual parasite stages. Parasite line was GFP-tagged (strain D10 ACP_leader-GFP) where apicoplast fluorescence is in green. DAPI staining is in blue while Pf-WRS is stained with Alexa594 (red). Pre-immune sera was used as negative control in these experiments.