Figure 5. TMPyP4 and hydrogen peroxide treatment result in similar fitness changes.

(A) Correlation plot of fitness differential values from the TMPyP4 screen and H₂O₂ screen. Genes in red (bottom left-hand corner) were found to increase sensitivity to both TMPyP4 and H₂O₂ when deleted (FD ≤−0.5). Genes in green (top right-hand corner) were found to decrease sensitivity to both TMPyP4 and H₂O₂ when deleted (FD ≥0.5). Blue lines denote FD thresholds. (B) Spot test for TMPyP4 and H₂O₂ sensitivity. Strains were grown to saturation in YEPD before a 5-fold serial dilution in water and spotting onto plates with or without 100 µM TMPyP4. Incubation was carried out at 30°C for 3 days. Strains from three different genetic backgrounds (W303, S288C and BY4741) were tested, as indicated on the left hand side and Table S1 in File S1.