Figure 4. Modulation of Compound 48/80-induced RBL-2H3 degranulation.

Compound 48/80 treatment of RBL- 2H3 cells induced a concentration dependent release of β-hexosaminidase. 30 minutes pre-incubation with 10 and 100 µM fexofenadine reduced β-hexosaminidase release in control cells and cells treated with 500 and 1000 µg Compound 48/80/ml (figure A). Carebastine pretreatment did not show an effect on Compound 48/80 stimulated RBL-2H3 cells (figure B). Results are a representative example of 3 independent experiments and expressed as mean ± SEM (4 wells per condition, *P<0.05).