Figure 2. Cleavage of HBV S mRNA sequence (substrate s38) by human RNase P in the presence of different EGSs.

No EGS was added to the reaction mixture in lane 1. EGS S-C386-C (10 nM) (lane 2), S-C386 (2 nM) (lane 3), and S-SER (10 nM) (lane 4) were incubated with [³²P]-labeled S RNA substrate (20 nM) and human RNase P (2 units) at 37°C in a volume of 10 µl for 45 minutes in buffer A (50 mM Tris, pH 7.4, 100 mM NH₄Cl, and 10 mM MgCl₂). Experimental details can be found in Materials and Methods.