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. 2013 Jun 12;8(6):e66166. doi: 10.1371/journal.pone.0066166

Table 1. Novel primer and probe sequences and PCR conditions.

Target Forward primer Reverse primer Hybridisation probe PCR conditions
mecC out frame 5′-TGTTGTAGCAATGTTCACAC-3′ 5′-CAAGCACTTAATATCAACGC-3′ n.a. 2 min at 95°C/30 cycles (30 sec at 95°C, 30 sec at 55°C, 2 min at 72°C)/5 min at 72°C
mecC 5′-ATTAATTGGACCCACATAACC-3′ n.a. 5′-AAAGCCGTGTTTATCCATTGAACGAAG-3′, spotted on the microarray n.a.
mecC 5′-TATAGTTAAATGAAGATCTTTTCCG-3′ n.a. 5′-GGTTTTAAGGTATCCATTGCAAATACTTATGAC-3′, spotted on the microarray n.a.
blaZ-M10/0065 5′-CAGCATTGCGCTACTTATAG-3′ n.a. 5′-AAGCGTTTTGCATATGCTTCCACATTA-3′, spotted on the microarray n.a.
etD2 5′-TCAAGACACCACTAGAAGTC-3′ 5′-CGTTTTCAGCTAATCGTGC-3′ n.a. 2 min at 94°C/35 cycles (30 sec at 94°C, 30 sec at 52°C, 30 sec at 72°C)/5 min at 72°C