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. 2013 Jun 12;8(6):e66077. doi: 10.1371/journal.pone.0066077

Figure 6. TPC2 knockdown inhibited neuronal differentiation from mouse ES cells derived neural progenitors.

Figure 6

(A) and (B) RNA was harvested at indicated time points during neural differentiation in control and TPC2 knockdown ES cells and analyzed for expression of Mash1 (A) and Nurr1 (B) by qRT-PCR. (C) Immunofluorescent analysis of Tuj1 expression (TujI, Green; DAPI, blue) in control and TPC2 knockdown ES cells harvested at indicated time points during differentiation (Scale bar = 200 µm). Data quantification was presented as Tuj1-positive cells/DAPI-stained cells ± S.E., n = 5 (40–50 cells per experiment). The * symbols indicate the results of t Test analysis, p<0.05. (D) Cell lysates were harvested at indicated time points during neural differentiation in both control and TPC2 knockdown cells, and analyzed for expression of Tuj1 by western blot analyses.