Table 1. Details of mouse monoclonal antibodies (mAb) used for flow cytometry.
mAb | Specificity | Isotype | Conjugation | Cellular expression | Source |
CC8 | CD4 | IgG2a | Alexa Fluor® 647 | Helper T cells [64] | AbD Serotec1 |
CC63 | CD8 | IgG2a | R-phycoerythrin | Cytotoxic T cells [65] | AbD Serotec1 |
TÜK4 | CD14 | IgG2a | Alexa Fluor® 647 | Monocytes, macrophages [66] | AbD Serotec1 |
CC21 | CD21 | IgG1 | R-phycoerythrin | B cells [67] | AbD Serotec1 |
AKS1 | CD335 (NKp40) | IgG1 | R-phycoerythrin | Natural killer cells [39] | AbD Serotec1 |
MM1A | CD3 | IgG1 | n/a | Pan-T cells [68] | VMRD Inc.2 |
GB21A | γδ T cell receptor | IgG2b | n/a | Pan-γδ T cells [69] | VMRD Inc.2 |
P3.6.2.8.1 | Isotype control | IgG1 | R-phycoerythrin | n/a | eBioscience3 |
eBM2a | Isotype control | IgG2a | R-phycoerythrin | n/a | eBioscience3 |
eBM2a | Isotype control | IgG2a | Alexa Fluor® 647 | n/a | eBioscience3 |
VPM21 | Isotype control | IgG1 | n/a | n/a | MRI4 |
VPM22 | Isotype control | IgG2b | n/a | n/a | MRI4 |
AbD Serotec, Kidlington, UK;
VMRD Inc., Pullman, WA;
eBioscience, San Diego, CA;
Moredun Research Institute; n/a = not applicable.