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. 2013 Jun 12;8(6):e65915. doi: 10.1371/journal.pone.0065915

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© 2013 Naranjo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Percent identity matrix created by Clustal 2.1 for subolesin coding sequences in I. scapularis tick cell lines (1, IDE8; 3, ISE6) and tick strains (2, Wikel; 4, OSU; 5, NY; 6, RI1; 7, RI2). (B) Exon/intron/exon junction sequences in subolesin and akirin genes. The exon/intron/exon junction sequences are highly conserved in all organisms (consensus sequence). Human (akirin2; AC_000138), tick (JX898528) and fly (AE014296) subolesin/akirin gene exon/intron/exon junction sequences are shown. Nucleotides that differ from consensus sequence are shown in lower case letters. Nucleotides conserved among all three sequences are underlined. Abbreviations: E, exon; I, intron.