Figure 1. Effects of ROCK1 or ROCK2 deletion on cell adhesion rate. WT and ROCK1^−/− cells (A) and WT and ROCK2^−/− cells (B) were seeded at a density of 1 × 10⁵/well in 10% FBS DMEM and left to adhere for the indicated times. After removal of unattached cells, attached cell viability was measured by MTT assay. Assays were performed in triplicate. Attached cell viability was expressed as percentage of the cells attaching for 120 min. No significant difference on cell adhesion rate was detected among WT, ROCK1^−/− and ROCK2^−/− cells. Error bars represent standard deviation *p < 0.05 vs. WT under the same condition.