Figure 3. ROCK1 deletion, but not ROCK2 deletion, reduces doxorubicin-induced impairment of focal adhesion formations. Representative images of focal adhesion formation revealed by p-FAK staining (green) of individual WT, ROCK1^−/− and ROCK2^−/− cells at baseline and after treatment with 3 μM doxorubicin for 16 h. Rhodamine-phalloidin staining for F-actin (red), and DAPI staining for nuclei (blue) are shown in merged images. Typical focal adhesions are indicated with white arrows. Bar, 50 μm.